rabbit anti-human mmp2  (Insight Biotechnology Ltd)

Journal: International Journal of Molecular Sciences

Article Title: Suppressive Potential of Ethanolic Extracts of Parkia speciosa Hassk. Empty Pods Against Colon Cancer Cell Migration and Invasion

doi: 10.3390/ijms27042072

Figure Lengend Snippet: Western blot showing the effects of the PSET on the abundance of proteins associated with the downstream EMT pathway. Representative Western blot image showing the amount of MMP2, MMP9 and GAPDH proteins in control (untreated cells) and treatment (PSET) groups. The molecular weight (MW) of each protein is provided. Quantitative analysis of protein levels following Western blot analysis. Data in histograms are presented as the means ± SDs from three independent experiments ( n = 3), with asterisks indicating statistical differences: * p < 0.05.

Article Snippet: The primary antibodies used were rabbit polyclonal anti-human MMP2 (#4022), MMP9 (#3852), N-cadherin (#4061), and GAPDH (#2118) (dilution 1:500; Cell Signaling Technology, Danvers, MA, USA).

Techniques: Western Blot, Control, Molecular Weight

Journal: Molecular Systems Biology

Article Title: Synovial matrix turnover controls immune cell spatial patterning in inflammation resolution

doi: 10.1038/s44320-025-00149-7

Figure Lengend Snippet: Following differential expression analysis between newly defined AMP1-like fibroblast clusters of the Alivernini dataset, DEGs were prioritised based on DEG conservation between differential expression analysis methods edgeR and MAST, conservation in a newly performed MAST analysis of the Wei mouse dataset of STIA, log2FC and adjusted P values. ( A ) Ranks of the top ten prioritised matrisomal DEGs in active RA/remission lining and sub-lining fibroblasts. ( B ) Network analysis of protein abundances and correlations in Graphia. Each protein is represented by a dot. Protein clusters are grouped by colour, with orange proteins corresponding to proteins more abundant in RA, green proteins more abundant in OA, and purple and yellow proteins in neither. ( N = 12 for OA and N = 10 for RA). ( C ) Heatmaps of protein abundance for proteins found to be overrepresented in RA (orange) and OA (green) samples. Stars denote a P value < 0.05 through t test. ( N = 12 for OA and 10 for RA). ( D ) Abundance of Collagen-I and VI protein in OA and RA patient samples ( N = 12 and N = 10, respectively), each dot represents the abundance of a tryptic peptide. For Collagen-I chains Col1a1 and Col1a2 have been grouped. For Collagen-VI, chains Col6a1, Col6a2 and Col6a3 have been grouped. ( E ) The ratio of semi-tryptic collagen-I peptide abundance compared to the average tryptic peptide abundance, this is as an indicator of degradation for Collagen-I and -VI in OA and RA. Each dot represents the ratio for a single semi-tryptic peptide. ( N = 12 for OA and 10 for RA). ( F ) Supporting data from RA synovial biopsy ( N = 8) dataset. Semi-tryptic ratio of collagen-I and –VI based on relevant spectral counts. Tryptic spectral counts ( G ) and semi-tryptic ratio ( H ) from the same dataset as ( F ) but separated into lymphoid ( N = 4) and myeloid ( N = 4) endotypes for grouped collagen-I and –VI. P values for t tests are labelled above each dot plot. ( I ) Dot plot of genes for type VI collagen chains and its processing regulators in each AMP1-like fibroblast cluster, split by disease activity status, coloured by scaled average expression, and sized by percentage of expressing cells. Red asterisks separate comparisons that led to genes being classified as DEGs. ( J ) Suggested mathematical model for out-of-phase oscillations in MMP2/MMP9 and TIMP3/TIMP4 expression, the subsequent anabolism potential of the tissue they induce, and its putative impact on collagen VI deposition. ( F : collagen I vs collagen VI in RA synovium P = 0.0011, G : lymphoid vs myeloid counts for collagen I P = 0.056 and for collagen VI P = 0.1056). Exact P values are given unless they are below 0.0001 which is beyond the calculation limit of the software used. .

Article Snippet: Rabbit anti-human MMP2 , Atlas Antibodies , HPA001939.

Techniques: Quantitative Proteomics, Activity Assay, Expressing, Software

Journal: Molecular Systems Biology

Article Title: Synovial matrix turnover controls immune cell spatial patterning in inflammation resolution

doi: 10.1038/s44320-025-00149-7

Figure Lengend Snippet: ( A ) Immunofluorescent staining was used to characterise the spatial distribution of COL4A1, COL6A1, CHP, and CD14. QuPath was used to visualise immunofluorescent imaging of COL6A1 (magenta), CHP (green), CD14 (cyan), COL4A1 (red) in section JRP122 (representative of 3 OA and 3 RA sections). ( B – D ) Degraded collagen VI peptides with either increased abundance in RA (red) or OA (blue) were mapped back to 50–100 amino acid sections of the α1 ( B ), α2 ( C ), and α3 ( D ) chain structures. The frequency of cleavage sites within these sections was plotted in both bars and heatmaps. Corresponding heatmaps for these same sections were generated from Manchester proteome cleave (MPC) ( https://www.manchesterproteome.manchester.ac.uk/#/MPSC ) with a susceptibility score cut-off of 0.75 for MMP2/9 cleavage and the frequency of these were predicted cleavage sites were plotted in green. Stars indicate experimentally validated MMP9 cleavage sites. .

Article Snippet: Rabbit anti-human MMP2 , Atlas Antibodies , HPA001939.

Techniques: Staining, Imaging, Generated

Journal: iScience

Article Title: Autocrine and paracrine effects of MDK promote lymph node metastasis of cervical squamous cell carcinoma

doi: 10.1016/j.isci.2024.110077

Figure Lengend Snippet: Cervical cancer cell-derived MDK promotes LNM in vitro and in vivo (A) Transwell assay (migration and invasion) of MS751 NC and MS751 MDK cells. Scale bar = 10 μm. (B) Transwell assay(migration) of C33a shMDK-1, C33a shMDK-3 and C33a NC cells. Scale bar = 10 μm. (C) Expression of MMP2 and MMP9 was analyzed by WB. MS751 MDK and MS751 NC cells (left panel), C33a shMDK-1, C33a shMDK-3 and C33a NC cells (right panel). (D) CCK8 experiment detected the proliferation ability of HLECs cultured by conditioned medium of MS751 MDK/MS751 NC cells. (E) Typical diagram of sprouting of HLECs pretreated with conditioned medium of MS751 MDK/MS751 NC cells. Scale bar = 10 μm. (F) HLECs were pretreated with conditioned medium of MS751 MDK/MS751 NC cells to investigate the effects of MDK on the tube formation of HLECs. Representatives are shown (left panel). Total branch points and total tuber length were calculated (right panel). Scale bar = 10 μm. (G) CCK8 experiment detected the proliferation ability of HLECs cultured by conditioned medium of C33a shMDK-1/C33a shMDK-3/C33a NC cells. (H) HLECs were pretreated with conditioned medium of C33a shMDK-1/C33a shMDK-3/C33a NC cells to investigate the effects of MDK on the tube formation of HLECs. Representatives are shown (left panel). Total branch points and total tuber length were calculated (right panel). Scale bar = 10 μm. (I) Immunofluorescence staining of Occludin (blue), Claudin5 (red) and DAPI (green) in HLECs cultured by conditioned medium. Scale bar = 100 μm. (J) The expressions of ZO-1, Claudin5 and Occludin in HLECs cultured by conditioned medium were analyzed by WB. (K) Typical images of gross anatomy of hindfoot lymphatic drainage model constructed by MS751 MDK/MS751 NC cells; Black arrows: the visible enlarged lymph nodes. (L) The number of metastatic lymph nodes in MS751 NC group and MS751 MDK group were compared. (M) Expression of LYVE-1 in transplanted tumor of foot pad was validated by IHC analysis. Scale bar = 20 μm. (N) The number of lymphatic vessels per visual field in MS751 NC group and MS751 MDK group were compared. The data represent the median with 95%CI. Each experiment was independently repeated at least three times. ∗ p < 0.05 , ∗∗ p < 0.01 , ∗∗∗ p < 0.001 .

Article Snippet: Rabbit anti-human MMP2 antibody , Beyotime, China , Cat# AF1420.

Techniques: Derivative Assay, In Vitro, In Vivo, Transwell Assay, Migration, Expressing, Cell Culture, Immunofluorescence, Staining, Construct

Journal: iScience

Article Title: Autocrine and paracrine effects of MDK promote lymph node metastasis of cervical squamous cell carcinoma

doi: 10.1016/j.isci.2024.110077

Figure Lengend Snippet:

Article Snippet: Rabbit anti-human MMP2 antibody , Beyotime, China , Cat# AF1420.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, ATP Assay, Western Blot, RNA Sequencing, Expressing, Plasmid Preparation, Software, Microscopy, SYBR Green Assay, Flow Cytometry